Production of Recombinant α-Galactosidases in Thermus thermophilus

Thermus Thermophilus -galactosidases in Α Production of Recombinant

Thermoadaptation of -Galactosidase AgaB1 in Thermus thermophilus

The evolutionary potential of a thermostable -galactosidase, with regard to improved catalytic activity at high temperatures, was investigated by employing an in vivo selection system based on thermophilic bacteria. For this purpose, hybrid -galactosidase genes of agaA and agaB from Bacillus stearothermophilus KVE39, designated agaA1 and agaB1, were cloned into an autonomously replicating Therm...

Enhancing Allosteric Inhibition in Thermus thermophilus Phosphofructokinase

The coupling between the binding of the substrate Fru-6-P and the inhibitor phospho(enol)pyruvate (PEP) in phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus is much weaker than that seen in a PFK from Bacillus stearothermophilus. From the crystal structures of Bacillus stearothermophilus PFK (BsPFK) the residues at positions 59, 158, and 215 in BsPFK are located on the...

Transcription strategy of Thermus thermophilus bacteriophage φYS40

From the Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182 Russia, Mathematical Biosciences Institute, The Ohio State University, Columbus, OH 43210, Waksman Institute for Microbiology, Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, Institute for Information Transmission Problems, Russian Academy of ...

Transferable denitrification capability of Thermus thermophilus.

Laboratory-adapted strains of Thermus spp. have been shown to require oxygen for growth, including the model strains T. thermophilus HB27 and HB8. In contrast, many isolates of this species that have not been intensively grown under laboratory conditions keep the capability to grow anaerobically with one or more electron acceptors. The use of nitrogen oxides, especially nitrate, as electron acc...